Large-Scale Clinical Expansion of Mesenchymal Stem Cells in the GMP-Compliant, Closed Automated Quantum® Cell Expansion System: Comparison with Expansion in Traditional T-Flasks
نویسندگان
چکیده
Abbreviations: GMP: Good Manufacturing Practice; BM: Bone Marrow; HSC: Hematopoietic Stem Cells; MSC: Mesenchymal Stem/Stromal Cells; HCT: Hematopoietic Cell Transplantation; NK: Natural Killer; NOD/SCID: Non-Obese Diabetic/Severe Combined Immunodeficient mice; aGVHD: acute Graft Versus Host Disease; LTCG: Laboratory of Cell and Gene Therapy; EBMT: European group for Blood and Marrow Transplantation; CBT: Cord Blood Transplantation; ATMP: Advanced Therapeutic Medicinal Product; PBS: Phosphate-Buffer Saline; HAS: Human Serum Albumin; DMSO: Dimethylsulfoxyde; IC: Intracapillary; DMEM: Dulbecco’s Modified Eagles Medium; DT: doubling time; FBS: Fetal bovine serum; NEAA: Non Essential Amin Acid; β-ME: β−mercaptoethanol; PBMC: Peripheral Blood Mononuclear Cells; MNC: Mononuclear cells; hESC: Human Embryonic Stem Cells; FCS: Fetal Calf Serum; PL: Platelet Lysate
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